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September JoellaThype Rabu, 07 September Jaimecaula Rabu, 07 September Donnysmors Rabu, 07 September Junkoarraw Rabu, 07 September HilaryTrerb Rabu, 07 September Debralok Kamis, 08 September Jimmiedaw Kamis, 08 September Timothybeivy Kamis, 08 September Joycefef Kamis, 08 September TrevorStymn Kamis, 08 September Prestonhom Kamis, 08 September Williamrah Kamis, 08 September Bessienag Kamis, 08 September AmandaFus Kamis, 08 September Anniechoob Kamis, 08 September Prestonhom Jumat, 09 September TerriDooke Jumat, 09 September Kevinhet Jumat, 09 September DavidPhacy Jumat, 09 September Arleneslify Jumat, 09 September Pamelasam Jumat, 09 September Ronaldlog Jumat, 09 September WnwxAgine Jumat, 09 September MoniqueWen Jumat, 09 September LindaSwilt Jumat, 09 September Gracedak Jumat, 09 September LindaMep Jumat, 09 September SandraNeork Jumat, 09 September Vernadug Jumat, 09 September Jeffreyfeata Jumat, 09 September Berthalap Jumat, 09 September DavidNex Jumat, 09 September Jybetemetoona Jumat, 09 September JosephAsype Jumat, 09 September Lailapougs Jumat, 09 September StarTuh Sabtu, 10 September Richardgog Sabtu, 10 September Natashaidern Sabtu, 10 September MicheleSitty Sabtu, 10 September Rebeccates Sabtu, 10 September Julienox Sabtu, 10 September Joycehex Sabtu, 10 September MarthaLoulp Sabtu, 10 September Brucerom Sabtu, 10 September Dorcascep Sabtu, 10 September CindyDuh Sabtu, 10 September Rebeccarible Sabtu, 10 September Andreaorigo Sabtu, 10 September MarieNolla Sabtu, 10 September NellieSah Minggu, 11 September CharlesNathe Minggu, 11 September GlendaVal Minggu, 11 September JoyceTilky Minggu, 11 September LillianAmili Minggu, 11 September Katrinagon Minggu, 11 September Carolbut Minggu, 11 September Lindavaf Minggu, 11 September Juliejep Minggu, 11 September MarlenePum Minggu, 11 September ScottJet Minggu, 11 September PartnerTuh Senin, 12 September Sarahshuth Senin, 12 September Nbqzslele Senin, 12 September EddieFuelo Senin, 12 September DavidPhacy Senin, 12 September Jessehem Senin, 12 September Albinautisp Senin, 12 September Jameshit Senin, 12 September DavidJew Senin, 12 September JasonApony Senin, 12 September Waynesom Selasa, 13 September RonaldGrise Selasa, 13 September LatexTuh Rabu, 14 September Amandasweam Rabu, 14 September KizExast Rabu, 14 September RonaldGrise Rabu, 14 September DavidJew Rabu, 14 September Meghangom Rabu, 14 September TiffanyVed Rabu, 14 September Antonioriree Rabu, 14 September Mahalialer Rabu, 14 September Curr Opin Genet Dev Mol Cell Biochem Additionally, miR, which can enhance the formation of capillary-like structures Tiwari et al.

Taken together, the increased expressions of miRa and , and the decreased expression of miR induced by ISMN may play a role in angiogenesis. In conclusion, our study revealed that ISMN could promote angiogenesis in embryonic development of zebrafish via regulating vegf-a , kdrl , and pdgfr.

Moreover, by using HUVECs, we found that the decreased apoptosis, down-regulated miR level and up-regulated levels of miR as well as miRa may also contributed to angiogenesis.

These findings deepen our understanding of the angiogenic ability of cardiovascular drugs in treating CHD. However, limitations exist in the present work. It remains to know whether inhibition and overexpression of the angiogenic genes could influence angiogenesis. Moreover, clinical trials should also be designed for therapeutic evaluation of the pro-angiogenic therapy with ISMN. Thus, future studies should be employed to further clarify the specific angiogenic mechanisms mediated by ISMN.

Open menu Brazil. Genetics and Molecular Biology. Open menu. Text EN Text English. E-mail: Introduction Coronary heart disease CHD is still the major cause of death in the world even if the past 20 years witnessed a dramatic decline Colquhoun et al. Material and Methods Zebrafish embryo collection and drug administration Tg flk:eGFP zebrafish embryos, in which vascular endothelial cells are fluorescently stained with the enhanced green fluorescent protein eGFP , were generated by natural pairwise mating and were maintained at Results ISMN accelerated blood vessel formation To figure out the effects of cardiovascular medicines on angiogenesis, we first examined the vessel formation of zebrafish embryos.

A Fluorescence images of 24 hpf zebrafish embryos treated with cardiovascular drugs for 24 h. B The number of ISVs in zebrafish embryos. C The length of ISVs in zebrafish embryos. Figure 2 Effects of nitrates on angiogenesis of ISVs. A Fluorescence images of zebrafish embryos at 24 hpf treated with DMSO control and different nitrates. B The number of ISVs in zebrafish embryos treated with nitrates. C The length of ISVs in zebrafish embryos treated with nitrates.

A Relative expression of vegf-a. B Relative expression of vegfr-2 kdrl. C Relative expression of vegfr-3 flt4. A fli1a expression. B pdgfr expression. L-ISMN, 0. DMEM treatment was used as control. Csont T and Ferdinandy P Cardioprotective effects of glyceryl trinitrate: Beyond vascular nitrate tolerance.

Emanueli C and Madeddu P Therapeutic angiogenesis: Translating experimental concepts to medically relevant goals. Jones DS and Greene JA The decline and rise of coronary heart disease: understanding public health catastrophism.

Kruzliak P, Kovacova G and Pechanova O Therapeutic potential of nitric oxide donors in the prevention and treatment of angiogenesis-inhibitor-induced hypertension. Liu F and Patient R Genome-wide analysis of the zebrafish ets family identifies three genes required for hemangioblast differentiation or angiogenesis.

Supplementary Material The following online material is available for this article: Table S1 Primers for genes involved in angiogenesis in zebrafish. Associate Editor: Igor Schneider. Publication Dates Publication in this collection 24 July Date of issue History Received 13 July Accepted 28 May License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License type CC-BY , which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.

Send correspondence to Yongwen Qin. All authors read and approved the final version. Figures 7.